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jinnjuice solution from Jul 19, 2012 at 1:42 PM worked for me!

jctorrente, i think i figured out. if you assigned permissions to "NETWORK SERVICE" account, then try changing your "ASP.NET v4.0" application pool's identity from "ApplicationPoolIdentity" to "NETWORK SERVICE".

Here's my setup and a re-explanation:

BugNet v1.5.265
Win O/S Server 2008 R2
SQL 2008
dB Name = BugNet, and the "NETWORK SERVICE" account is set as the db_owner (in Properties > User Mapping)

<connectionStrings>
        <clear/>
        <add name="BugNET" connectionString="server=.\SQLExpress;database=BugNet;Trusted_Connection=True;uid={username};pwd={password}" providerName="System.Data.SqlClient" /> 
    </connectionStrings>

For IIS 7.5:

1. click Applications Pools
2. highlight ASP.NET v4.0
3. click Advanced Settings... from Actions pane on the right
4. Under Process Model section, edit the Identity property
5. Change the Built-in account to "NetwrokService" (instead of "ApplicationsPoolIdentity")